Magnetic 3D Cell Culturing

Magnetic 3D Cell Culturing 

The core technology is the magnetization of cells with NanoShuttle™-PL. The cells can be aggregated with magnetic forces, either by levitation or printing, to form structurally and biologically representative 3D models in vitro.

 
The advantages of magnetic cell culture include:

  •         Mimicking native tissue environment
  •         Rapid 3D model formation within hours
  •         No specialized equipment, media, or artificial substrate
  •         Easy to handle / no sample loss
  •         Allows co-culture

With magnetized spheroids, solution addition and removal is made easy by using magnetic force to hold them in a stationary position during aspiration, thereby limiting spheroid loss. Spheroids can also be picked up and transferred between vessels using magnetic tools such as the MagPen™.


NanoShuttle™-PL consists of gold, iron oxide, and poly-L-lysine. NanoShuttle™-PL magnetizes cells by electrostatically attaching to cell membranes during an overnight static incubation. Magnetized cells will appear peppered with dark nanoparticles after incubation. NanoShuttle™-PL will stay attached to the cell membrane for up to 8 days, at which point it’s released into the 3D culture. NanoShuttle™-PL is biocompatible, having no effect on metabolism, proliferation, and inflammatory stress, and even encouraging proliferation in 3D. Additionally, it does not interfere with experimental techniques, such as fluorescence, or Western blotting.

Productlist

Item: 655841

96 WELL BIOPRINTING KIT, µCLEAR®, BLACK, NANOSHUTTLE, 600 µL, SPHEROID DRIVE, HOLDING DRIVE, 96 WELL MICROPLATE, CELL-REPELLENT SURFACE

Item: 657840

6 WELL BIO-ASSEMBLER KIT, CLEAR, NANOSHUTTLE 600 µL, LEVITATING DRIVE, HOLDING DRIVE, 6 WELL MICROPLATE, CELL-REPELLENT SURFACE

Item: 662840

24 WELL BIO-ASSEMBLER KIT, CLEAR, NANOSHUTTLE 600 µL, LEVITATING DRIVE, HOLDING DRIVE, 24 WELL MICROPLATE, CELL-REPELLENT SURFACE

Item: 655840

96 WELL BIOPRINTING KIT, CLEAR, NANOSHUTTLE 600 µL, SPHERIOD DRIVE, HOLDING DRIVE, 96 WELL MICROPLATE, CELL-REPELLENT SURFACE

Item: 781840

384 WELL BIOPRINTING KIT, CLEAR, NANOSHUTTLE 600 µL, SPHEROID DRIVE, HOLDING DRIVE, 384 WELL MICROPLATE, CELL-REPELLENT SURFACE

Item: 781846

384 WELL BIO ASSAY KIT, CLEAR, NANOSHUTTLE 600 µL, 6 WELL + 384 WELL DRIVES, 6 WELL + 384 WELL MICROPLATES, CELL-REPELLENT SURFACE, 96 WELL MASTERBLOCK

Item: 781849

384 WELL BIO ASSAY KIT + IMAGING SYSTEM, NANOSHUTTLE 600 µL, 6 WELL + 384 WELL DRIVES, 6 WELL + 384 WELL MICROPLATES, CELL-REP. SURF., 96 WELL MASTERBLOCK, IMAG. SYS., LIGHT PAD

Related Downloads

F074093_White Paper GBO PerkinElmer
White Paper / PDF, 822 KB
Whitepaper Magnetic 3D Cell Culturing
White Paper / PDF, 1 MB
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